The staphylococcal accessory regulator, SarA, is an RNA-binding protein that modulates the mRNA turnover properties of late-exponential and stationary phase Staphylococcus aureus cells

dc.contributor.authorAnderson, Kelsi L., PhD Morrison, John M. Beenken, Karen E. Smeltzer, Mark S. Dunman, Paul M.
dc.date.accessioned2019-01-09T19:09:25Z
dc.date.available2019-01-09T19:09:25Z
dc.date.issued2012-03
dc.description.abstractThe modulation of mRNA turnover is gaining recognition as a mechanism by which Staphylococcus aureus regulates gene expression, but the factors that orchestrate alterations in transcript degradation are poorly understood. In that regard, we previously found that 138 mRNA species, including transcripts coding for the virulence factors protein A (spa) and collagen-binding protein (cna), are stabilized in a sarA-dependent manner during exponential phase growth, suggesting that SarA directly or indirectly affects the RNA turnover properties of these transcripts. Herein, we expanded our characterization of the effects of sarA on mRNA turnover during late-exponential and stationary phases of growth. Results revealed that the locus affects the RNA degradation properties of cells during both growth phases. Further, using gel mobility shift assays and RIP-Chip, it was found that SarA protein is capable of binding mRNA species that it stabilizes both in vitro and within bacterial cells. Taken together, these results suggest that SarA post-transcriptionally regulates S. aureus gene expression in a manner that involves binding to and consequently altering the mRNA turnover properties of target transcripts.en_US
dc.identifier.citationMorrison JM, Anderson KL, Beenken KE,Smeltzer MS and Dunman PM (2012) The staphylococcal accessory regulator, SarA, is an RNA-binding protein that modulates the mRNA turnover properties of late-exponential and stationary phase Staphylococcus aureus cells. Front.Cell.Inf.Microbio. 2:26. doi: 10.3389/fcimb.2012.00026en_US
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3417590/
dc.identifier.urihttp://hdl.handle.net/20.500.11987/334
dc.language.isoen_USen_US
dc.publisherFrontiers in Cellular and Infection Microbiologyen_US
dc.subjectStaphylococcus aureusen_US
dc.subjectSarAen_US
dc.subjectRNA degradationen_US
dc.subjectRNA stabilityen_US
dc.subjectBacterial proteinsen_US
dc.subjectGene expression regulation, bacterialen_US
dc.titleThe staphylococcal accessory regulator, SarA, is an RNA-binding protein that modulates the mRNA turnover properties of late-exponential and stationary phase Staphylococcus aureus cellsen_US
dc.typeArticleen_US
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