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Characterizing the Antibacterial Properties and Transcriptional Alternations Induced by Lemongrass Oil in Staphylococcus aureus

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dc.contributor.author Christensen, Collin J.
dc.contributor.author Anderson, Kelsi L
dc.date.accessioned 2018-06-25T01:44:25Z
dc.date.available 2018-06-25T01:44:25Z
dc.date.issued 2017
dc.identifier.citation Christensen CJ and Anderson KL. Characterizing the Antibacterial Properties and Transcriptional Alternations Induced by Lemongrass Oil in Staphylococcus aureus. The Journal of Experimental Microbiology and Immunology. JEMI+. 2017; 3:57-62. en_US
dc.identifier.uri https://jemi.microbiology.ubc.ca/node/137
dc.identifier.uri http://hdl.handle.net/20.500.11987/42
dc.description.abstract Essential oils have risen in popularity as “all natural” alternatives used to treat a myriad of conditions. To begin to elucidate the antibacterial properties of essential oils, we tested the effectiveness of lemongrass oil (LGO), tea tree oil (TTO), and willow bark extract (WBE) against Staphylococcus aureus growth. To do so, a Methicillin-Resistant strain of Staphylococcus aureus (USA300) was exposed to each oil using disk diffusion assays. Of the oils, LGO had the greatest zone of inhibition. The Minimum Inhibitory Concentration (MIC) of both LGO and citral (the primary chemical component of LGO) was determined in macro-broth cultures; exposure to increased concentrations of each resulted in dramatic cell death as determined by cell growth assays. To begin to determine the molecular mechanisms underlying the observed antibacterial effects, we exposed cells to a sub-inhibitory concentration of citral and hybridized the RNA to Affymetrix GeneChips®. Transcripts differentially affected in citral- versus mocktreated cells represent virulence factors, hypothetical proteins, and intergenic regions. Taken together, these results demonstrate that LGO exhibits antibacterial properties against a highly pathogenic bacterial species that is exceedingly resistant to the currently available antibiotics. en_US
dc.description.sponsorship Concordia University Intramural Research Grant; Council on Undergraduate Research and the American Society for Microbiology. en_US
dc.language.iso en_US en_US
dc.publisher The Journal of Experimental Microbiology & Immunology+ en_US
dc.subject Staphylococcus aureus Antimicrobial properties lemongrass oil en_US
dc.title Characterizing the Antibacterial Properties and Transcriptional Alternations Induced by Lemongrass Oil in Staphylococcus aureus en_US
dc.type Article en_US


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