Faculty, administrators and staff of the Bryan College of Health Sciences are encouraged to submit their professional scholarship to the Scholarly Works Archives. This might include, but is not limited to, peer-reviewed journal articles; theses or dissertations; abstracts of books or book chapters; conference papers or presentations; technical reports; white papers; abstracts or presentations from College symposia; professional website content. Selected student works will be included upon the recommendation of a faculty member.
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Authors will be required to sign a Non-Exclusive Distribution license for each item submitted to the institutional repository. Full-text of the work will be uploaded into the repository if the author owns the copyright to the work, or has written permission from the publisher to add the work to an archive. If archiving permission is not granted, links will be made to the publisher content for the article.
(Frontiers in Cellular and Infection Microbiology, 2012-03) Anderson, Kelsi L., PhD
Morrison, John M.
Beenken, Karen E.
Smeltzer, Mark S.
Dunman, Paul M.
The modulation of mRNA turnover is gaining recognition as a mechanism by which Staphylococcus aureus regulates gene expression, but the factors that orchestrate alterations in transcript degradation are poorly understood. In that regard, we previously found that 138 mRNA species, including transcripts coding for the virulence factors protein A (spa) and collagen-binding protein (cna), are stabilized in a sarA-dependent manner during exponential phase growth, suggesting that SarA directly or indirectly affects the RNA turnover properties of these transcripts. Herein, we expanded our characterization of the effects of sarA on mRNA turnover during late-exponential and stationary phases of growth. Results revealed that the locus affects the RNA degradation properties of cells during both growth phases. Further, using gel mobility shift assays and RIP-Chip, it was found that SarA protein is capable of binding mRNA species that it stabilizes both in vitro and within bacterial cells. Taken together, these results suggest that SarA post-transcriptionally regulates S. aureus gene expression in a manner that involves binding to and consequently altering the mRNA turnover properties of target transcripts.